Journal: Emerging Microbes & Infections

Article Title: Treponema pallidum protein Tp0136 promotes angiogenesis to facilitate the dissemination of Treponema pallidum

doi: 10.1080/22221751.2024.2382236

Figure Lengend Snippet: Tp0136 promoted angiogenesis in vivo and in vitro . (a) Effect of Tp0136 protein and antibody on blood vessels in lesions. Red arrows indicate the blood vessels. The bar graph indicates the number of the blood vessels. Scale bars = 100 mm. (b) Effect of Tp0136 on cell migration using transwell migration assay. The bar graph indicates the crystal violet area. Scale bars = 100 mm. The experiment was repeated three times independently for each group. (c) Effect of Tp0136 on spheroid-based sprouting. The bar graph indicates the number of sprouting. Scale bars = 100 mm. The experiment was repeated three times independently for each group. (d) Effect of Tp0136 on tubule formation. Tp0136 (10 mg/mL) was coincubated with HMEC-1 cells for 6 h, after which tubule formation was stained using Calcein AM. Bar graphs indicate the number of nodes, the number of meshes, and the total branching length. Scale bars = 500 mm. The experiment was repeated three times independently for each group. (e-f) Three-dimensional microfluidic angiogenesis system of angiogenesis processed by Tp0136. (g) Effect of Tp0136 on three-dimensional angiogenesis. The bar graph indicates the number and the average length of angiogenic sprouts. Phalloidin-iFluor 555-red, DAPI-blue. Scale bars = 100 mm. The experiment was repeated three times independently for each group. Data are expressed as the mean ± SD from three independent experiments. Student's t test was used to compare data between two groups. One-way ANOVA was used to compare the mean values of three or more groups with one independent variable. * P vs. Control, *, P <0.05, **, P <0.01, ***, P <0.001.

Article Snippet: Human microvascular endothelial cell line-1 (HMEC-1) (Procell Life Science & Technology, China) was maintained in HMEC-1 cell-specific medium (Procell Life Science & Technology, China) in a humidified incubator at 37 ℃ and 5% CO2 for 3 days, and the medium was changed every 24 h. For the Transwell migration assay, to uphold the assay's reproducibility, a total of 8 × 10 4 cells were seeded in the chamber for 3 days.

Techniques: In Vivo, In Vitro, Migration, Transwell Migration Assay, Staining, Control

Journal: Emerging Microbes & Infections

Article Title: Treponema pallidum protein Tp0136 promotes angiogenesis to facilitate the dissemination of Treponema pallidum

doi: 10.1080/22221751.2024.2382236

Figure Lengend Snippet: Tp0136 increased vascular permeability with angiogenesis. (a) Effect of Tp0136 on vascular permeability detected using Evans blue-bovine serum albumin. Left: Vascular permeability in HMEC-1 cells stimulated with different concentrations of Tp0136 for 6 h. Right: Vascular permeability in HMEC-1 cells incubated with 10 mg/mL Tp0136 for different durations. The experiment was repeated three times independently for each group. (b) Effect of Tp0136 on vascular permeability detected using three-dimensional angiogenesis analysis. The pseudo-colored fluorescent images after 0 and 8 min after the addition of the dextran solutions. Time is indicated in h, min, and s. Data are expressed as the mean ± SD from three independent experiments. One-way ANOVA was used to compare mean values of three or more groups with one independent variable. * P < 0.05, ** P < 0.01, *** P < 0.001, ns; nonsignificant difference.

Article Snippet: Human microvascular endothelial cell line-1 (HMEC-1) (Procell Life Science & Technology, China) was maintained in HMEC-1 cell-specific medium (Procell Life Science & Technology, China) in a humidified incubator at 37 ℃ and 5% CO2 for 3 days, and the medium was changed every 24 h. For the Transwell migration assay, to uphold the assay's reproducibility, a total of 8 × 10 4 cells were seeded in the chamber for 3 days.

Techniques: Permeability, Incubation

Journal: Emerging Microbes & Infections

Article Title: Treponema pallidum protein Tp0136 promotes angiogenesis to facilitate the dissemination of Treponema pallidum

doi: 10.1080/22221751.2024.2382236

Figure Lengend Snippet: Expression of PI3K/AKT signaling pathway in Tp0136-induced angiogenesis. (a) GO analysis of the first 3000 differentially expressed genes after Tp0136 treatment in HMEC-1 cells. (b) KEGG pathway analysis of the first 3000 differentially expressed genes after Tp0136 treatment in HMEC-1 cells. (c) Expression of the PI3K, p-PI3K, AKT, and p-AKT proteins in HMEC-1 cells stimulated with different concentrations of Tp0136 revealed using western blotting. The bar graphs on the right indicate the ratios of the phosphorylated proteins versus the total proteins. The experiment was repeated three times independently for each group. (d) Expression of the PI3K, p-PI3K, AKT, and p-AKT proteins in HMEC-1 cells stimulated with Tp0136 for different durations using western blotting. The experiment was repeated three times independently for each group. Data are expressed as the mean ± SD from three independent experiments. Two-way ANOVA was used to compare the mean values of three or more groups with two independent variables. * P vs. Control, * P < 0.05, ** P < 0.01, *** P < 0.001, ns; nonsignificant difference.

Article Snippet: Human microvascular endothelial cell line-1 (HMEC-1) (Procell Life Science & Technology, China) was maintained in HMEC-1 cell-specific medium (Procell Life Science & Technology, China) in a humidified incubator at 37 ℃ and 5% CO2 for 3 days, and the medium was changed every 24 h. For the Transwell migration assay, to uphold the assay's reproducibility, a total of 8 × 10 4 cells were seeded in the chamber for 3 days.

Techniques: Expressing, Western Blot, Control